Adolescent alcohol exposure, pain, and synaptic function at BLA inputs onto prelimbic neurons
Principal Investigator(s): View help for Principal Investigator(s) J Daniel Obray, Medical University of South Carolina
Version: View help for Version V1
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Project Citation:
Obray, J Daniel. Adolescent alcohol exposure, pain, and synaptic function at BLA inputs onto prelimbic neurons. Ann Arbor, MI: Inter-university Consortium for Political and Social Research [distributor], 2025-03-21. https://doi.org/10.3886/E223721V1
Project Description
Summary:
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This dataset covers mechanical and thermal sensitivity in PV-Cre rats (RRID:RRRC_00773) beginning in adolescence and continuing into adulthood. The rats were tested weekly for eight weeks. After the first test, half the rats began adolescent intermittent ethanol vapor exposure, which continued for 4 weeks. Rats then underwent surgery to express channelrhodopsin in the BLA, and a viral cre-depedent mCherry tag in the prelimbic cortex. Retrobeads were injected in the ventrolateral periaqueductal gray. This allowed for slice electrophysiology recordings from prelimbic parvalbumin interneurons and prelimbic pyramidal neurons projecting to the ventrolateral periaqueductal gray. After recovering from the surgery, rats underwent an inflammatory pain challenge using carrageenan injected into the hindpaw. After assessing the effects of alcohol exposure in conjuction with the pain challenge on mechanical and thermal sensitivity slice electrophysiology recordings were made from neurons in the prelimbic cortex. Parameters of interest were intrinstic excitability and BLA-driven excitatory/inhibitory balance, AMPA/NMDA ratio, and aEPSCs at both parvalbumin interneurons and pyramidal neurons projecting from the prelimbic cortex to the ventrolateral periaqueductal gray.
Funding Sources:
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NIH (AA019967);
NIH (AA007474);
NIH (AA030193)
Scope of Project
Subject Terms:
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AIE;
adolescent alcohol;
pain;
allodynia;
parvalbumin interneurons;
PVINs;
Prelimbic Cortex;
ventrolateral periaqueductal gray;
vlPAG;
PrL
Collection Date(s):
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7/23/2021 – 12/31/2023 (September 2021 - December 2023)
Universe:
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PV-Cre rats (RRID:RRRC_00773) from a colony maintained at the Medical University of South Carolina in Charleston, SC.
Data Type(s):
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experimental data
Collection Notes:
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Excitation/Inhibition balance data represent polysynaptic neurotransmission.
AMPA/NMDA ratios and aEPSCs represent monosynaptic (TTX + 4-AP) neurotransmission.
Intrinsic excitability data were recorded in the presence of picrotoxin and kynurenic acid.
AMPA/NMDA ratios and aEPSCs represent monosynaptic (TTX + 4-AP) neurotransmission.
Intrinsic excitability data were recorded in the presence of picrotoxin and kynurenic acid.
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